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Biological Physics (iPoLS) Seminar: Rohit Bhargava (UIUC) "Progress in chemical imaging to record structure and chemistry in biological materials"

Event Type
Lecture
Sponsor
Physics Department
Location
Beckman Institute 3269
Date
Oct 14, 2022   2:00 pm  
Speaker
Rohit Vaidya
Contact
Sharlene Denos
E-Mail
denos@illinois.edu
Phone
217-244-0672
Views
43
Originating Calendar
Physics - Biological Physics (iPoLS) Seminar

Infrared (IR) spectroscopic imaging allows the recording of molecular and structural properties of complex samples without the need for prior information on composition, dyes or stains. The spatial and spectral domains are coupled, presenting both opportunities and challenges for recording high-content, high-fidelity data that determine image quality. We first provide an optical theoretic and information theoretic approach to present a roadmap for the development of optical microscopy-based imaging. We show how the spatial quality is related to spectral data and provide explicit relationships to devise imaging configurations at performance limits. We show how molecular content over large areas can be recorded by new high speed microscopy configurations and present rigorous approaches to extending molecular understanding with linear and circularly polarized light. Finally, we present a detailed theoretical understanding of image formation in nanoscale spectroscopic imaging using an atomic force microscope in contact mode. A new method, based on null deflection of the cantilever, is introduced to provide high quality data.

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