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Special Biological Physics Seminar: "Revealing Principles of Subcellular RNA Localization"

Event Type
Seminar/Symposium
Sponsor
Deaprtment of Physcis
Location
144 Loomis
Date
Feb 28, 2020   2:00 pm  
Speaker
Furqan M. Fazal, Stanford University School of Medicine, Stanford CA
Contact
Marjoire Gamel
E-Mail
mgamel@illinois.edu
Phone
217-333-3762
Views
56
Originating Calendar
Physics - Biological Physics (CPLC/iPoLS) Seminar

The human body is composed of trillion of cells, which are the building blocks of life. Each cell is highly organized, and contains RNAs that code for proteins and serve regulatory roles. The location of an RNA species within a cell can dictate its folding1, editing, splicing, translation, degradation, binding partners, catalytic activity, and even the fate of the protein that it encodes. However, characterizing the RNA contents of cellular compartments that cannot be biochemically isolated is challenging. Here I introduce APEX‐seq2, a method for RNA sequencing based on the direct proximity labeling of RNA using the peroxidase enzyme APEX2. APEX‐seq in nine distinct subcellular locales produced a nanometer-resolution spatial map of the human transcriptome, revealing extensive patterns of localization for diverse RNA classes and transcript isoforms. I uncovered a radial organization of the nuclear transcriptome, which is gated at the inner surface of the nuclear pore for cytoplasmic export of processed transcripts. I identified two distinct pathways of messenger RNA localization to mitochondria, each associated with specific sets of transcripts for building complementary macromolecular machines within the organelle. APEX‐seq should be widely applicable to many systems and model organisms, enabling comprehensive investigations of the dynamic spatial transcriptome.

 

  1. Sun L*, Fazal FM*, Li P*, Broughton JP, Lee B, Tang L, Huang W, Kool ET, Chang HY, Zhang QC. RNA structure maps across mammalian cellular compartments. Nature Structural and Molecular Biology (NSMB), 26, 322-330 (2019).
  2. Fazal FM*, Han S*, Parker KR, Kaewsapsak P, Xu J, Boettiger AN, Chang HY, Ting AY. Atlas of subcellular RNA localization revealed by APEX-seq. Cell, 178, 473–490 (2019).
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