Dr. Goldstrohm and his team seek to understand how gene expression is regulated at the post-transcriptional level. Regulation of translation, degradation and localization of mRNAs contributes to the enormous dynamic range of protein expression. Sequence specific RNA-binding factors, both protein and small RNAs, play a central role in mRNA regulation. Our research focuses on Pumilio proteins, which are cytoplasmic RNA-binding proteins that control diverse processes including stem cell fate and neurological functions, such as behavior and memory formation. Novel findings show that dysfunction of Pumilio proteins contributes to neurodegeneration, epilepsy, movement disorders, intellectual disability, infertility, and cancer. In this presentation, we explore the impact of Pumilio proteins on gene expression and discuss insights into the mechanisms of RNA binding, translational control, and mRNA destruction.