The next C3.ai Digital Transformation Institute Colloquia on Digital Transformation Science will be Thursday, April 15 at 3:00 p.m. U.S. Central time via Zoom. Presenting "AI Enabled Deep Mutational Scanning of Interaction between SARS-CoV-2 Spike Protein S and Human ACE2 Receptor" will be Diwakar Shukla from the University of Illinois at Urbana-Champaign.
Registration is required to attend this event.
Abstract: The rapid and escalating spread of SARS-CoV-2 poses an immediate public health emergency. The viral spike protein S binds ACE2 on host cells to initiate molecular events that release the viral genome intracellularly. Soluble ACE2 inhibits entry of both SARS and SARS-2 coronaviruses by acting as a decoy for S binding sites, and is a candidate for therapeutic and prophylactic development. Deep mutational scans is one of the approaches that could provide such a detailed map of protein-protein interactions. However, this technique suffers from several issues such as experimental noise, expensive experimental protocol and lack of techniques that could provide second or higher-order mutation effects. In this talk, we describe an approach that employs a recently developed platform, TLmutation, that could enable rapid investigation of sequence-structure-function relationship of proteins. In particular, we employ a transfer learning approach to generate high-fidelity scans from noisy experimental data and transfer the knowledge from single point mutation data to generate higher-order mutational scans from the single amino-acid substitution data. Using deep mutagenesis, variants of ACE2 will be identified with increased binding to the receptor binding domain of S at a cell surface. We plan to employ the information from the preliminary mutational landscape to generate the high order mutations in ACE2 that could enhance binding to S protein. We also aim to investigate this problem using distributed computing approaches to understand the underlying physics of the spike protein and ACE2 interaction.
