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BIOE Seminar Series: Professor Patrick La Riviere

Event Type
Seminar/Symposium
Sponsor
Department of Bioengineering
Virtual
wifi event
Date
Feb 3, 2021   12:00 - 12:50 pm  
Speaker
Professor Patrick La Riviere, University of Chicago
Registration
Register in advance for this meeting
Contact
Rose Smith
E-Mail
rjsmth@illinois.edu
Views
63
Originating Calendar
Bioengineering calendar

3D and 4D Computational Imaging of Molecular Orientation with Multiview Polarized Fluorescence Microscopy

Abstract: If a fluorophore is rigidly attached to a molecule of interest, then the orientation of the fluorophore can report valuable information about the orientation and diffusive behavior of the molecule. To measure and interpret this information in living cells we have developed a new set of techniques for imaging and reconstructing the position and orientation of fluorophores throughout a three-dimensional sample. These techniques consist of a multiview, polarized-illumination light sheet microscope coupled with novel computational models that relate the measured intensities to the orientation distribution function of fluorophores at each voxel. These new models, when expanded in a basis of complex exponentials and spherical harmonics, give insight into the capabilities of the imaging system and also provide a framework for efficient solution of the inverse problem.

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