Contrast-free Peripheral Perfusion Imaging in the Diabetic Ischemic Mouse Hindlimb
Abstract: This study focuses on the potential of power-Doppler ultrasound (PD-US) in monitoring muscle perfusion changes related to peripheral artery disease (PAD). PAD is marked by arterial blockages due to atherosclerotic plaques, often assessed through ankle-brachial index (ABI) tests, but challenges arise in interpretation, especially in older patients, and in detecting endothelial dysfunction. We aimed to assess PD-US sensitivity and specificity in detecting muscle perfusion changes in a unilateral ischemic hindlimb model for healthy and diabetic mice. Our investigation covered eight groups, considering gender and activity level. Using a 24 MHz ultrasound transducer, we applied spatial registration before PCA clutter filtering, effectively isolating perfusing blood signal. Findings revealed extended angiogenic recovery times in diabetic mice, reduced by exercise conditioning. PD-US presents potential for non-invasive monitoring of peripheral muscle perfusion changes, suggesting applicability in monitoring PAD progression and treatment responses with minor software modifications.
Bio: With a foundation rooted in academic achievement, I obtained my BS in Physics and MS in Medical Radiation Engineering in Iran. Embarking on a journey of academic pursuit, I commenced my Ph.D. in Bioengineering at UIUC in 2019. Presently, I am engaged as a Research Assistant in Dr. Insana's Lab, while also serving as a Teaching Assistant for Bioe302. My research focuses on the innovative realm of Perfusion Imaging utilizing ultrasound technology.