Title: Defense exam: Transient intra- and extra-cellular thermometry as probes of thermogenesis
Research summary:
Temperature is a fundamental thermodynamic property affecting every biochemical reaction in the cellular milieu. Thermometry in tissues has proven useful in understanding thermoregulatory neuronal circuits and cancer metabolism. In contrast, intracellular temperature changes are relatively less explored. In this work, we first describe an intracellular thermometry technique that we used to measure transient temperature changes during stimulated mitochondrial thermogenesis in neurons of Aplysia californica. Our measurements demonstrate the utility of transient intracellular thermometry in better understanding the thermochemistry of mitochondrial metabolism. Using insights from intracellular thermometry, we theoretically examine the expected temperature changes at intra- and extra-cellular regions. For physiologically relevant thermogenic reactions, extracellular thermometry is better suited, especially in tissues or organs. To this end, we develop a wireless temperature measurement technique using magnetostriction based sensors that can potentially measure temperatures at tissue length-scales remotely. Overall, this work advances intra- and extra-cellular thermometry techniques that potentially provide unprecedented insight into thermogenesis in cells
Time: Oct 19, 2020 03:30 PM Central Time (US and Canada)
Join Zoom Meeting
https://illinois.zoom.us/j/94045114027?pwd=VjIvR2J4MGpLeTEyeXg0cnVyQXZBQT09
Meeting ID: 940 4511 4027
Password: 551681
