Title: "Ultra‐high resolution structural and molecular imaging of cells and tissues."
Since Robert Hooke’s first description of a cell in Micrographia 350 years ago, microscopy has played an important role in understanding the rules of life. Far-field fluorescence microscopy is a powerful approach in biological and biomedical research due to its live cell compatibility and molecular specificity. A major hurdle over the last century has been the limited resolution due to the diffraction of light. Super-resolution microscopy methods, such as single-molecule localization microscopy, overcome this fundamental barrier to improve the resolution limit (250-600 nm, lateral-axial) down to a few nanometers. The development and application of SMLM majorly focus on fixed cells in thin samples and cellular structures that lie on/close to the coverslip surface and therefore, the profound impact of SMLM on biophysical, medical, and biological research has yet to fully unfold. Dr. Huang will discuss some of his team’s most recent projects which explore newly available sensors/devices such as high-speed sensors and deformable mirrors, analytical methods such as deep learning, and novel optical instrumentation to allow SMLM imaging in live cells and tissue specimens. He will show the capabilities of these new imaging systems in revealing the fine details of subcellular structures from a diverse set of biological systems including viruses, bacteria, yeasts, mammalian cells, and brain sections.