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Biochemistry Seminar: Dr. Jiajie Diao (University of Cincinnati), "Studying SNARE-mediated membrane fusion at the single vesicle level"

Event Type
Seminar/Symposium
Topics
biochemistry, biological sciences, biophysics, chemistry, molecular biology, nanotechnology, protein structure
Sponsor
Dr. Kai Zhang, Department of Biochemistry
Location
Charles G. Miller Auditorium, B102 Chemical and Life Science Laboratory
Date
Feb 21, 2020   12:00 pm  
Speaker
Dr. Jiajie Diao
Contact
Sherry Unkraut
E-Mail
sunkraut@illinois.edu
Phone
217-333-2013
Views
40
Originating Calendar
Biochemistry Department Seminars

Membrane fusion is one of the most important cellular processes by which two initially distinct lipid bilayers merge their hydrophobic cores, resulting in one interconnected structure. The so-called SNARE proteins play a central role in many fusion processes and they regulated by several accessory proteins. In order to study SNARE-mediated membrane fusion, in vitro protein reconstitution assays involving ensemble FRET have been used over a decade. Recently, we have developed single-vesicle-based FRET approaches to overcome shortcomings of ensemble assays for studying the dynamics and kinetics of SNARE-mediated membrane fusion. Moreover, we also created an optogenetic tool to control the conformation of SNAREs and studied the role of protein clusters in membrane fusion.

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