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CPLC Special Seminar: "Integrative Structure and Functional Anatomy of a Nuclear Pore Complex."

Event Type
Seminar/Symposium
Sponsor
Department of Physics
Location
276 Loomis
Date
Feb 5, 2018   1:00 pm  
Speaker
Seung Joong Kim, University of California, San Francisco
Contact
Marjorie Gamel
E-Mail
mgamel@illinois.edu
Phone
217-333-3762
Views
70
Originating Calendar
Physics - Physics of Living Cells Seminar

Despite the central role of Nuclear Pore Complexes (NPCs) as gatekeepers of RNA and protein transport between the cytoplasm and nucleoplasm, their large size and dynamic nature have impeded a full structural and functional elucidation. Here, we have determined a subnanometer precision structure for the entire 552-protein yeast NPC by satisfying diverse data, including primarily stoichiometry, a cryo-electron tomography map, and chemical cross-links. The structure reveals the NPC’s functional elements in unprecedented detail. The NPC is built of sturdy diagonal columns to which are attached connector cables, imbuing both strength and flexibility, and tying together all other elements of the NPC, including membrane-interacting regions and RNA processing platforms. Inwardly-directed anchors create a high density of transport factor-docking Phe-Gly repeats in the NPC’s central channel, organized in distinct functional units. Taken together, this integrative structure allows us to rationalize the architecture, transport mechanism, and evolutionary origins of the NPC.

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