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Biochemistry Seminar: Dr. Bryce E. Nickels (Rutgers University), "Use of metabolic effectors as non-canonical initiating nucleotides provides an "ab initio" mechanism for RNA 5’ capping"

Event Type
Seminar/Symposium
Topics
academic, biochemistry
Sponsor
Dr. Raven Huang
Location
Charles G. Miller Auditorium (B102 CLSL)
Date
Mar 9, 2018   12:00 pm  
Speaker
Dr. Bryce E. Nickles
Cost
Free
Contact
Sherry Unkraut
E-Mail
sunkraut@illinois.edu
Phone
217-333-2013
Views
47
Originating Calendar
Biochemistry Department Seminars

We have established that the metabolic effectors nicotinamide adenine dinucleotide (NAD+), reduced nicotinamide adenine dinucleotide (NADH), and 3'-desphospho-coenzyme A enable a direct regulatory connection between metabolism and gene expression by serving as non-canonical initiating nucleotides (NCINs) for transcription initiation. We have comprehensively defined the sequence-determinants for NCIN-mediated initiation by bacterial RNA polymerase with NAD+, and developed methods for the detection and quantification of NCIN-capped RNA both in vitro and in vivo. Together, these studies identify a previously unknown, “ab initio,” mechanism for RNA 5’ capping with nucleoside-containing metabolites

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