Materials Research Laboratory

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Materials Science and Engineering Colloquium

Event Type
Materials Science and Engineering Department
100 Materials Science and Engineering Building
Feb 25, 2013   4:00 pm  
Sarah Heilshorn, Department of Materials Science and Engineering, Stanford University
Originating Calendar
MatSE Seminars

"Design of self-assembling materials for cell transplantation" - Stem cell transplantation is a promising therapy for a myriad of debilitating diseases and injuries; however, current delivery protocols are inadequate. Transplantation by direct injection, which is clinically preferred for its minimal invasiveness, commonly results in less than 5% cell viability, greatly inhibiting clinical outcomes. We demonstrate that mechanical membrane disruption results in significant acute loss of viability at clinically relevant injection rates. As a strategy to protect cells from these damaging forces, we show that cell encapsulation within hydrogels of specific mechanical properties will significantly improve viability. Building on these fundamental studies, we have designed a reproducible, bio-resorbable, customizable hydrogel using protein-engineering technology. In our Mixing-Induced Two-Component Hydrogel (MITCH), network assembly is driven by specific and stoichiometric peptide-peptide binding interactions. By integrating protein science methodologies with simple polymer physics models, we manipulate the polypeptide chain interactions and demonstrate the direct ability to tune the network crosslinking density, sol-gel phase behavior, and gel mechanics. This is in contrast to many other physical hydrogels, where predictable tuning of bulk mechanics from the molecular level remains elusive due to the reliance on non-specific and non-stoichiometric chain interactions for network formation. Furthermore, our MITCH materials enable stem cell and growth factor encapsulation at constant physiological conditions ' a significant advantage over other commonly used hydrogels such as collagen and Matrigel. Through a series of in vitro and in vivo studies, we demonstrate that these materials may significantly improve transplanted stem cell retention.

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